Cytogenetic damage induced in human lymphocytes by four vanadium compounds and micronucleus analysis by fluorescence in situ hybridization with a centromeric probe.
Mutat Res
; 319(3): 205-13, 1993 Nov.
Article
en En
| MEDLINE
| ID: mdl-7694142
ABSTRACT
The genotoxicity of four vanadium compounds, sodium metavanadate (NaVO3), ammonium metavanadate (NH4VO3), sodium ortovanadate (Na3VO4) and vanadyl sulfate (SVO5), was evaluated in human lymphocyte cultures using structural and numerical chromosome aberrations, micronuclei, sister-chromatid exchanges and satellite chromosome associations as endpoints. These compounds were not found to increase the frequency of structural chromosome aberrations whereas a significant increase in numerical aberrations, micronuclei and satellite associations was found. Since these results could have been related to a possible mechanism of the action of vanadium as a mitotic spindle poison, the fluorescence in situ hybridization (FISH) technique was applied to the human lymphocyte micronucleus assay, by means of an alphoid centromere-specific DNA probe. The four vanadium salts showed a micronucleus percentage with positive signal (presence of centromere and thus of whole chromosome(s)) that was always higher than 68% at all doses tested. That confirmed the aneuploidogenic potentiality of vanadium.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Compuestos de Vanadio
/
Mutágenos
Límite:
Humans
/
Male
Idioma:
En
Revista:
Mutat Res
Año:
1993
Tipo del documento:
Article
País de afiliación:
Italia