Isolation and characterization of the mouse PDGF beta-receptor promoter.
Biochem Biophys Res Commun
; 210(1): 165-73, 1995 May 05.
Article
en En
| MEDLINE
| ID: mdl-7741738
ABSTRACT
The PDGF beta-receptor expression is tightly regulated during embryonic development and in several physiological and pathological situations. To determine the regulatory mechanism of the receptor, a 1.9 kb 5' flanking genomic fragment of the mouse PDGF beta-receptor gene was cloned and analyzed by functional promoter assays. The fragment was shown to exert promoter activity in the luciferase expression vector system in mouse NIH 3T3 fibroblast and NB41 neuroblastoma cell lines as well as rat ST15A cerebellar cell lines. Functional studies on deletion mutants revealed several putative regulatory sequences. The deletion mutants acted similarly in NB41 cells and in ST15A cells, both of neuronal origin, but differently in the NIH 3T3 fibroblasts. No TATA box was found in the analyzed promoter region, however, site directed mutagenesis of a CCAAT motif, located 60 basepair upstream of the transcriptional start site, almost completely abolished the promoter activity in all cell types.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Receptores del Factor de Crecimiento Derivado de Plaquetas
Límite:
Animals
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
1995
Tipo del documento:
Article
País de afiliación:
Suecia
Pais de publicación:
EEUU
/
ESTADOS UNIDOS
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ESTADOS UNIDOS DA AMERICA
/
EUA
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UNITED STATES
/
UNITED STATES OF AMERICA
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US
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USA