Oral immunization with recombinant Helicobacter pylori urease induces secretory IgA antibodies and protects mice from challenge with Helicobacter felis.
J Infect Dis
; 172(1): 161-72, 1995 Jul.
Article
en En
| MEDLINE
| ID: mdl-7797906
ABSTRACT
Helicobacter pylori, a gram-negative spiral bacterium, is the cause of chronic superficial (type B) gastritis and peptic ulcer disease. The urease enzyme of H. pylori was expressed as an inactive recombinant protein in Escherichia coli, purified as particulate structures of 550-600 kDa molecular mass with a diameter of approximately 12 nm. Given orally, 5 micrograms of urease with an appropriate mucosal adjuvant, such as the labile toxin of E. coli, protected 60%-100% of mice against challenge with virulent Helicobacter felis. Protection correlated with the level of secretory IgA antibodies against urease. Oral administration of antigen was as effective or better than intragastric administration. Parenteral injection of antigen or intragastric administration of high-dose antigen without adjuvant elicited serum IgG but no IgA antibodies and did not confer protection. Recombinant urease as an oral vaccine candidate deserves further investigation as an approach to the prevention of Helicobacter-induced chronic gastroduodenal diseases in humans.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ureasa
/
Inmunoglobulina A Secretora
/
Vacunas Bacterianas
/
Vacunas Sintéticas
/
Helicobacter pylori
/
Infecciones por Helicobacter
Límite:
Animals
/
Humans
Idioma:
En
Revista:
J Infect Dis
Año:
1995
Tipo del documento:
Article
País de afiliación:
Estados Unidos