[Genetic engineering of human insulin. IV. Development and optimization of an analysis system using reversed phase high pressure liquid chromatography]. / Genno-inzhenernyi insulin cheloveka. IV. Razrabotka i optimizatsiia sistemy analiza s pomoshch'iu obrashchenno-fazovoi vysokoéffektivnoi zhidkostnoi khromatografii.

ABSTRACT

The effectiveness of the RP HPLC application for the step-by-step analysis of the recombinant insulin production was studied. Properties of a number of commercial and experimental columns in different chromatographic conditions were considered. A three-dimension optimization of selectivity and resolution versus pH and ion strength was carried out. A mechanism of the resolution and selectivity control is suggested.