A recombinant cysteine-rich section of the Entamoeba histolytica galactose-inhibitable lectin is efficacious as a subunit vaccine in the gerbil model of amebic liver abscess.
J Infect Dis
; 171(3): 645-51, 1995 Mar.
Article
en En
| MEDLINE
| ID: mdl-7876611
ABSTRACT
The 170-kDa subunit of the galactose-inhibitable adherence lectin of Entamoeba histolytica mediates attachment to colonic mucins and host cells. The DNA fragment encoding the 170-kDa subunit was produced by polymerase chain reaction (PCR) and divided into four sections by restriction endonucleases. The third section (designated LC3, base pairs 2273-3397) encodes a cysteine-rich fusion protein that was recognized by adherence-inhibitory anti-lectin monoclonal antibodies and serum antibodies from 95% of subjects with amebic liver abscess. Immunization of gerbils with purified recombinant LC3-encoded protein (10 micrograms) with Titermax adjuvant elicited a high-titer serum anti-LC3 IgG antibody response and protective immunity against intrahepatic challenge with 0.5 x 10(6) virulent axenic trophozoites (strain HM1IMSS; 71% vaccine efficacy, P < .01). In summary, a recombinant cysteine-rich portion of the 170-kDa lectin subunit was highly antigenic, immunogenic, and effective as a subunit vaccine in an experimental animal model of amebic liver abscess.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Vacunas Sintéticas
/
Vacunas Antiprotozoos
/
Entamoeba histolytica
/
Galactosa
/
Lectinas
/
Absceso Hepático Amebiano
Límite:
Animals
Idioma:
En
Revista:
J Infect Dis
Año:
1995
Tipo del documento:
Article