High-performance liquid chromatographic determination of oxytetracycline in channel catfish (Ictalurus punctatus) muscle tissue.
Analyst
; 119(12): 2749-51, 1994 Dec.
Article
en En
| MEDLINE
| ID: mdl-7879888
ABSTRACT
A high-performance liquid chromatographic method for the determination of oxytetracycline in channel catfish muscle tissue is presented. Oxytetracycline is extracted three times from muscle tissue with an ethylenediaminetetraacetic acid disodium salt-McIlvaine buffer (pH 4.0) by using an Ultra Turrax. Analysis is carried out by using high-performance liquid chromatography and an acetonitrile-oxalic acid (0.05 mol 1(-1), pH 2.2) mixture (14 + 86, v/v) is used as mobile phase. Oxytetracycline is separated on a Lichrosorb RP-8 125 x 4.0 mm i.d. column and ultraviolet detection at 355 nm is used. The limit of quantification is 10 ng g-1 and the linearity, tested in the spiking range 20-500 ng g-1, is 0.9997. Recovery from muscle spiked at 20, 50, 100, 200 and 500 ng g-1 levels is in the range 70-80%. Precision, expressed as percentage relative standard deviation, is below 7%. The method is applied to muscle tissue from channel catfish fed on a medicated diet.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Oxitetraciclina
/
Músculo Esquelético
/
Carne
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
Revista:
Analyst
Año:
1994
Tipo del documento:
Article
País de afiliación:
Italia