Functional commitment to helper T cell lineage precedes positive selection and is independent of T cell receptor MHC specificity.
Immunity
; 1(4): 269-76, 1994 Jul.
Article
en En
| MEDLINE
| ID: mdl-7889414
ABSTRACT
Thymocyte differentiation proceeds from double positive CD4+CD8+ to single positive T cells. It has been proposed that this process occurs by an instructive or a stochastic mechanism. In this report, we show that in recombination-deficient mice (RAG-1-I-) constitutive expression of a CD8 transgene allows maturation of CD4+(CD8tg+) cells, which express mature levels of a transgenic class I-restricted T cell receptor, F5. Rescued F5+CD4+(CD8tg+) cells have equivalent levels of T cell receptor expression as CD8end+ cells, respond to cognate antigen and, upon stimulation, they exhibit a phenotype characteristic of CD4+ helper T cells. These data are consistent with a model of differentiation that predicts that thymocytes become functionally committed to a helper or cytotoxic lineage before the final step of positive selection and independently of MHC specificity of their T cell receptor.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Genes MHC Clase I
/
Receptores de Antígenos de Linfocitos T
/
Linfocitos T Colaboradores-Inductores
/
Proteínas de Homeodominio
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Immunity
Asunto de la revista:
ALERGIA E IMUNOLOGIA
Año:
1994
Tipo del documento:
Article
País de afiliación:
Reino Unido