Algorithms for automated characterization of cell populations in thick specimens from 3-D confocal fluorescence microscopy data.
J Microsc
; 173(Pt 2): 115-26, 1994 Feb.
Article
en En
| MEDLINE
| ID: mdl-7909568
ABSTRACT
Methods are presented for the automated, quantitative and three-dimensional (3-D) analysis of cell populations in thick, essentially intact tissue sections while maintaining intercell spatial relationships. This analysis replaces current manual methods which are tedious and subjective. The thick sample is imaged in three dimensions using a confocal scanning laser microscope. The stack of optical slices is processed by a 3-D segmentation algorithm that separates touching and overlapping structures using localization constraints. Adaptive data reduction is used to achieve computational efficiency. A hierarchical cluster analysis algorithm is used automatically to characterize the cell population by a variety of cell features. It allows automatic detection and characterization of patterns such as the 3-D spatial clustering of cells, and the relative distributions of cells of various sizes. It also permits the detection of structures that are much smaller, larger, brighter, darker, or differently shaped than the rest of the population. The overall method is demonstrated for a set of rat brain tissue sections that were labelled for tyrosine hydroxylase using fluorescein-conjugated antibodies. The automated system was verified by comparison with computer-assisted manual counts from the same image fields.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Algoritmos
/
Procesamiento de Imagen Asistido por Computador
/
Sustancia Negra
/
Microscopía Fluorescente
Límite:
Animals
Idioma:
En
Revista:
J Microsc
Año:
1994
Tipo del documento:
Article