Subcellular location and differential antibody specificity of arginase in tissue culture and whole animals.
Int J Dev Neurosci
; 12(4): 337-42, 1994 Jun.
Article
en En
| MEDLINE
| ID: mdl-7976488
ABSTRACT
Studies in man and other mammals have demonstrated the existence of two forms of arginase, a cytoplasmic form located primarily in liver and a mitochondrial form expressed in lesser amounts in a larger number of organs, but especially kidney. They appear to be encoded in different gene loci. Using a colloidal silica gradient separation technique, we have now located arginase in H4 cells, a rat hepatoma-derived line, to the cytoplasm and the arginase in human embryonic kidney-derived line, to the mitochondrion. Antibody prepared against A1 precipitates all the arginase from liver, 50% from kidney and none of the activity from human embryonic kidney (HEK) cells. An antibody prepared against partially purified All, by contrast, precipitates > 90% of arginase activity from HEK cells, half from kidney and virtually none from H4 cells or rat liver.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Arginasa
/
Fracciones Subcelulares
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Int J Dev Neurosci
Año:
1994
Tipo del documento:
Article