The aminoterminus of c-Raf-1 binds a protein kinase phosphorylating Ser259.
Biochem Biophys Res Commun
; 204(2): 841-8, 1994 Oct 28.
Article
en En
| MEDLINE
| ID: mdl-7980551
ABSTRACT
The kinase negative aminoterminal domain of c-Raf-1 expressed as glutathione S-transferase fusion protein was phosphorylated in vitro after treatment with lysates from A431 cells and subsequent in vitro protein kinase assay. This phosphorylation was independent of stimulation of the cells with EGF; it occurred exclusively on serine and was mapped to Ser259. The identical site of c-Raf-1 was phosphorylated in A431 cells by metabolic labelling in vivo. The kinase binding domain was mapped by various GST-Raf deletion mutants to c-Raf-1 aminoacid residues 181 to 255.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas Quinasas
/
Serina
/
Proteínas Proto-Oncogénicas
/
Proteínas Serina-Treonina Quinasas
Límite:
Humans
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
1994
Tipo del documento:
Article