Growth inhibition in vitro of murine mammary adenocarcinoma cells by heparin and chemically modified heparins.
Tumour Biol
; 15(5): 275-83, 1994.
Article
en En
| MEDLINE
| ID: mdl-7991988
ABSTRACT
Heparin, a highly sulfated polysaccharide used as an antithrombotic and anticoagulant, inhibits proliferation of several cell types. We have investigated the effect of heparin and chemically modified heparins on the growth of a cell culture of a murine mammary adenocarcinoma (M3). We found that heparin inhibited the proliferation of M3 cells growing either with or without 2% fetal calf serum (FCS) in a dose-dependent and reversible fashion. Several heparins with different anticoagulant properties showed a similar antiproliferative effect. Histological assays showed that heparin was internalized and appeared in cytoplasmic vesicules. O-desulfated, O/N-desulfated N-acetylated and N-desulfated heparins lost their antiproliferative activity, while N-desulfated N-acetylated heparin significantly inhibited cell proliferation with or without FCS. The finding of an antiproliferative action of N-desulfated N-acetylated heparin which does not show anticoagulant activity suggests a possible therapeutic role for this compound as an antineoplastic drug.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Heparina
/
Adenocarcinoma
/
Neoplasias Mamarias Experimentales
Límite:
Animals
Idioma:
En
Revista:
Tumour Biol
Asunto de la revista:
NEOPLASIAS
Año:
1994
Tipo del documento:
Article
País de afiliación:
Argentina