Metabolism of malate in bovine adrenocortical mitochondria studied by 13C-NMR spectroscopy.
Eur J Biochem
; 223(1): 51-9, 1994 Jul 01.
Article
en En
| MEDLINE
| ID: mdl-8033908
ABSTRACT
13C-NMR spectroscopy was used to study the metabolism of [13C]malate in bovine coupled adrenocortical mitochondria. The most apparent difference between the mitochondria from steroidogenic tissues and mitochondria from other tissues is the presence, in addition to the normal respiratory chain, of a second electron-transport system responsible for steroid hydroxylation. [13C]malate was synthesized from [13C]succinate by isolated adrenocortical mitochondria. The basic functional suspension consisted of oxygenated mitochondria to which were added ADP, inorganic phosphate (Pi) and [13C]malate, both in the absence or presence of the steroid substrate, deoxycorticosterone. These mitochondria synthesized [13C]citrate and [13C]pyruvate from [13C]malate. The 13C labeling of these two metabolites demonstrated an important role of the malic enzyme and the kinetics depended on the presence of the steroid substrate; the citric acid cycle was stopped during the hydroxylation pathway. The addition of cyanide, a strong inhibitor of the respiratory chain, confirmed an increased malic enzyme activity when hydroxylation occurred, since pyruvate was trapped by formation of a cyanohydrin. The relative enzymic activities of malic enzyme and isocitrate dehydrogenase were compared, both in the absence or presence of the steroid substrate, by supplementing the basic suspension with unlabeled exogenous metabolites, such as pyruvate or oxaloacetate.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Corteza Suprarrenal
/
Malatos
/
Mitocondrias
Límite:
Animals
Idioma:
En
Revista:
Eur J Biochem
Año:
1994
Tipo del documento:
Article
País de afiliación:
Francia