Separation of Lymnaea stagnalis hemocytes by density gradient centrifugation.
Dev Comp Immunol
; 18(1): 25-31, 1994.
Article
en En
| MEDLINE
| ID: mdl-8050613
ABSTRACT
A chelating anti-clumping (alpha-C) buffer allowed blood cells (hemocytes) of a gastropod, Lymnaea stagnalis to be separated by discontinuous Percoll density gradient centrifugation. The hemocytes of L. stagnalis were separated into five fractions, having a density lower than 10, 20, 30, 40, and 50% Percoll, respectively. Trypan blue exclusion assays showed viability of separated hemocytes to be between 81 and 89%. Cytospin preparations of these hemocytes were examined. Small cells were mainly observed at high densities; at lower densities medium and large hemocytes were also present. No absolute separation was achieved. Some density fractions were enriched for hemocytes with regard to the distributions of two endogenous lysosomal enzymes (alpha-naphthyl acetate esterase and acid phosphatase).
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Separación Celular
/
Centrifugación por Gradiente de Densidad
/
Hemocitos
/
Lymnaea
Límite:
Animals
Idioma:
En
Revista:
Dev Comp Immunol
Año:
1994
Tipo del documento:
Article
País de afiliación:
Países Bajos