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Application of genomic DNA subtyping by pulsed field gel electrophoresis and restriction enzyme analysis of plasmid DNA to characterize methicillin-resistant Staphylococcus aureus from two nosocomial outbreaks.
Branchini, M L; Morthland, V H; Tresoldi, A T; Von Nowakonsky, A; Dias, M B; Pfaller, M A.
Afiliación
  • Branchini ML; Study of Transmitted Diseases, Campinas State University, São Paulo, Brazil.
Diagn Microbiol Infect Dis ; 17(4): 275-81, 1993.
Article en En | MEDLINE | ID: mdl-8112042
ABSTRACT
Pulsed-field gel electrophoresis (PFGE) and restriction enzyme analysis of plasmid DNA (REAP) were applied to study the epidemiologic relationship among methicillin-resistant Staphylococcus aureus (MRSA) isolates from outbreaks in two hospitals in São Paulo, Brazil 82 MRSA isolates, 73 from a university hospital and nine from a general adult intensive care unit of a private hospital, were collected from 62 patients 95% of the MRSAs were also resistant to gentamicin and ciprofloxacin. REAP subtyping of both collections identified six different subtypes 55 (72.6%) MRSAs from the university hospital and nine isolates from the private hospital shared the same epidemic REAP subtype. Discrimination by restriction of genomic DNA with Sma I followed by PFGE enabled the identification of 14 DNA subtypes. Based on the combined REAP-genomic DNA subtype, the predominant subtype in the university hospital was A/A (44 isolates) whereas the epidemic subtype in the private hospital was A/M (seven isolates). The application of two typing methods showed better discrimination among MRSAs than did either method alone.
Asunto(s)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Staphylococcus aureus / Infección Hospitalaria / Brotes de Enfermedades / Resistencia a la Meticilina / Técnicas de Tipificación Bacteriana Límite: Humans País/Región como asunto: America do sul / Brasil Idioma: En Revista: Diagn Microbiol Infect Dis Año: 1993 Tipo del documento: Article País de afiliación: Brasil
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Staphylococcus aureus / Infección Hospitalaria / Brotes de Enfermedades / Resistencia a la Meticilina / Técnicas de Tipificación Bacteriana Límite: Humans País/Región como asunto: America do sul / Brasil Idioma: En Revista: Diagn Microbiol Infect Dis Año: 1993 Tipo del documento: Article País de afiliación: Brasil