Quantitative assessment of free-radical generation during ischemia and reperfusion in the isolated rabbit heart.
Coron Artery Dis
; 4(12): 1093-6, 1993 Dec.
Article
en En
| MEDLINE
| ID: mdl-8162240
ABSTRACT
BACKGROUND:
Several investigators have indirectly implicated oxygen free radicals in reperfusion injury following ischemia in the isolated rabbit heart.METHODS:
A quantitative assessment of free-radical production during ischemia and reperfusion was made using electron paramagnetic resonance (EPR) spectroscopy. Serial frozen (77 degrees K) tissue biopsies of the left ventricular wall in isolated rabbit hearts were performed during perfusion, ischemia, and reperfusion. These were pulverized into a fine powder that was filled into EPR tubes. EPR spectra of the tissue were recorded at 77 degrees K.RESULTS:
Three predominant signals were seen on EPRanalysis:
signal A, g = 2.028; signal B, g = 2.005, and signal C, g = 1.940. All three signals were present during the perfusion period and decreased in size during the period of ischemia. After reperfusion, all three signals doubled in size.CONCLUSION:
This study lends direct support to the theory that free radicals are generated during myocardial reperfusion and may result in reperfusion injury.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Oxígeno
/
Daño por Reperfusión Miocárdica
/
Isquemia Miocárdica
/
Ventrículos Cardíacos
Tipo de estudio:
Etiology_studies
Límite:
Animals
Idioma:
En
Revista:
Coron Artery Dis
Asunto de la revista:
ANGIOLOGIA
/
CARDIOLOGIA
Año:
1993
Tipo del documento:
Article