Human melanoma cells express functional endothelin-1 receptors.
Biochem Biophys Res Commun
; 201(1): 449-57, 1994 May 30.
Article
en En
| MEDLINE
| ID: mdl-8198607
ABSTRACT
Current evidence suggests that endothelium-derived factors enhance human melanoma vascular invasion. Therefore, we studied human melanoma cell expression of receptors to the endothelium-derived peptide, endothelin-1 (ET-1), and determined if they respond to ET-1 with proliferation and chemokinesis. Human metastatic melanoma cell lines were found to have specific, saturable, high affinity ET-1 binding. Northern analysis and competitive inhibition studies confirmed that melanoma cells express the ETB receptor isoform. Ten nanomolar ET-1 caused an 8.2 to 25.5-fold increase in intracellular free calcium. ET-1 was found to be a weak mitogen for melanoma cells, however, melanoma cell chemokinesis was significantly increased by ET-1. These data suggest that ET-1 may be involved in providing a chemokinetic and growth factor environment that enhances perivascular proliferation and invasiveness of melanoma cells.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Endotelinas
/
Receptores de Endotelina
/
Melanoma
Límite:
Humans
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
1994
Tipo del documento:
Article