Induction of inflammatory cytokines by Nocardia fractions.
Folia Biol (Praha)
; 39(5): 243-9, 1993.
Article
en En
| MEDLINE
| ID: mdl-8206169
ABSTRACT
NDCM and NLD fractions of Nocardia opaca cell walls were used for in vitro stimulation of human and porcine peripheral blood mononuclear cells. Inflammatory cytokines IL-1, IL-6, tumour necrosis factor (TNF) alpha and interferon (IFN) gamma were detected at the single-cell level using paraformaldehyde-fixed and saponin-permeabilized mononuclears stained with cytokine-specific antibodies and indirect immunofluorescence method. IL-1, IL-6 and TNF were produced by human monocytes stimulated for 2 h, IFN gamma-positive lymphocytes were detected later. IFN gamma was produced also by activated porcine lymphocytes. Cells expressing apoptotic features were found among blood mononuclears treated with Nocardia fractions.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Monocitos
/
Interleucinas
/
Interferón gamma
/
Factor de Necrosis Tumoral alfa
/
Esqueleto de la Pared Celular
/
Nocardia
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Folia Biol (Praha)
Año:
1993
Tipo del documento:
Article