Activin expression by cultured human retinal pigment epithelial cells.
Invest Ophthalmol Vis Sci
; 35(7): 2924-31, 1994 Jun.
Article
en En
| MEDLINE
| ID: mdl-8206710
ABSTRACT
PURPOSE:
To determine whether human retinal pigment epithelial (hRPE) cells produce activin, a growth factor in the transforming growth factor beta family, and to characterize growth regulatory effects of activin on retinal pigment epithelium.METHODS:
mRNA expression was examined using polymerase chain reaction with primers specific for the beta A and beta B chains of activin and by slot blot analysis with a probe specific for the beta A chain. Protein localization was determined immunocytochemically using antibodies specific for the beta A chain of activin and intact activin A. The effect of activin A on DNA synthesis was studied by measuring (3H) thymidine incorporation after cells were exposed to recombinant human activin A (rhA). Growth regulatory effects of rhA on hRPE cells were examined with cell growth assays.RESULTS:
beta A mRNA was expressed constitutively in 8/8 cells lines tested. beta B mRNA was not expressed in any of the six cell lines tested but was expressed in human ovarian granulosa cell controls. Positive immunostaining was observed for both the beta A chain and intact activin A. (3H) thymidine incorporation was inhibited 44% (P < 0.025), 45% (P < 0.025), and 44% (P < 0.015) when RPE cells were exposed to 100 ng/ml rhA and grown in serum-free medium, medium with 0.5% serum, and 1% serum, respectively. Cell growth was inhibited 33.2% (P = 0.0001) after RPE cells were exposed to 100 ng/ml rhA for 8 days.CONCLUSIONS:
These results suggest that activin A can act as an autocrine-paracrine growth regulator in RPE cells and may help control cellular growth in ocular development and proliferative eye disease.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Epitelio Pigmentado Ocular
/
ARN Mensajero
/
Inhibinas
Límite:
Humans
Idioma:
En
Revista:
Invest Ophthalmol Vis Sci
Año:
1994
Tipo del documento:
Article