Characterization and partial purification of the Croatian national standard Dermatophagoides pteronyssinus allergen extract.
Allergy
; 48(6): 454-9, 1993 Aug.
Article
en En
| MEDLINE
| ID: mdl-8238802
ABSTRACT
Lyophilized Dermatophagoides pteronyssinus (Der p) allergen extract (AE) and partially purified Der p extract (PAE) were prepared and characterized. Partial purification of AE was performed by gel filtration on Sephadex G-100 and Sephacryl S-300. Crossed immunoelectrophoresis (CIE) disclosed the same precipitating lines in AE and PAE preparations. The relative potencies of AE and PAE were determined and compared with the WHO International Standard for Der p by the RAST inhibition method. The potencies were 6.5 x 10(5) IU and 1.5 x 10(6) IU, respectively. Biologic standardization by quantitative skin testing was performed with AE (20 selected patients) and PAE (12 patients). Median Ch was calculated by linear regression analysis (log-log model). One ampoule of AE contained 65,300 BU and 1 ml (vial) of PAE contained 166,000 BU. Der p AE could serve as a croatian national standard for further production of Der p allergenic extracts.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Alérgenos
/
Polvo
/
Ácaros
Límite:
Adolescent
/
Adult
/
Aged
/
Animals
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Female
/
Humans
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Male
/
Middle aged
Idioma:
En
Revista:
Allergy
Año:
1993
Tipo del documento:
Article
País de afiliación:
Croacia
Pais de publicación:
DENMARK
/
DINAMARCA
/
DK