[The detection of human DNA in cell hybrids by the polymerase chain reaction with universal primers: the species specificity of the amplified DNA]. / Vyiavlenie DNK cheloveka v kletochnykh gibridakh metodom polimeraznoi tsepnoi reaktsii s universal'nymi praimerami: vidospetsifichnost' amplifitsirovannoi DNK.

ABSTRACT

The human DNA detection method is developed on the basis of DNA cross-hydridization of the amplification products obtained by the universally primed polymerase chain reaction (UP-PCR) technique. These PCR products are characterized by species-specificity in hybridization assay. Two somatic cell hybrids "human x Chinese hamster" supposed to contain the human DNA, according to selection procedure, were analysed by this method. As a result, the presence of human DNA, unable to be tested by cytological techniques, have been proven. The amplified human DNA can be mapped by this method.