Your browser doesn't support javascript.
loading
Molecular cloning of human amidophosphoribosyltransferase.
Iwahana, H; Oka, J; Mizusawa, N; Kudo, E; Ii, S; Yoshimoto, K; Holmes, E W; Itakura, M.
Afiliación
  • Iwahana H; Otsuka Department of Clinical and Molecular Nutrition, University of Tokushima, Japan.
Biochem Biophys Res Commun ; 190(1): 192-200, 1993 Jan 15.
Article en En | MEDLINE | ID: mdl-8380692
The cDNA of human amidophosphoribosyltransferase (EC 2.4.2.14, ATase), which is the supposed regulatory allosteric enzyme of de novo purine nucleotide biosynthesis, has been cloned from human hepatoma (HepG2) cDNA library. The predicted open reading frame encodes a protein of 517 amino acids with a deduced molecular weight (Mr) of 57,398, which is consistent with the molecular mass of 56 kDa on SDS-polyacrylamide gel electrophoresis (SDS-PAGE) of the ATase subunit purified from human placenta. The derived amino acid sequence exhibits 93, 82, 41, 37, and 33% identity with the sequences of rat, chicken, Bacillus subtilis, Escherichia coli, and Saccharomyces cerevisiae ATases, respectively. Southern blot analysis suggested that the ATase gene exists as multiple copies. ATase mRNA (3.5 kb) is ubiquitously expressed in various human tissues. Comparison with rat and chicken ATases showed that two cysteine residues for an iron-sulfur cluster were conserved. Four consensus phosphorylation sites for cAMP-dependent protein kinase were found.
Asunto(s)
Buscar en Google
Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Amidofosforribosiltransferasa Límite: Female / Humans / Pregnancy Idioma: En Revista: Biochem Biophys Res Commun Año: 1993 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos
Buscar en Google
Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Amidofosforribosiltransferasa Límite: Female / Humans / Pregnancy Idioma: En Revista: Biochem Biophys Res Commun Año: 1993 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos