Identification of a linear neutralization site within the third variable region of the feline immunodeficiency virus envelope.
J Virol
; 67(8): 4742-9, 1993 Aug.
Article
en En
| MEDLINE
| ID: mdl-8392611
ABSTRACT
Synthetic peptides have been used to map linear B-cell epitopes of the third variable (V3) region of the feline immunodeficiency virus (FIV) external membrane glycoprotein gp120. The analysis of sera from naturally and experimentally FIV-infected cats by Pepscan and enzyme immunoassay with four partially overlapping peptides evidenced three antibody-binding domains, two of which mapped in the carboxyl-terminal half of V3. In particular, the V3.3 sequence (Gly-392-Phe-413) turned out to be important for in vitro neutralization of the virus in that the peptide inhibited the FIV-neutralizing activity of pooled immune cat sera, and on the other hand, cat sera raised against this peptide effectively neutralized FIV infectivity for Crandell feline kidney cells.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Gatos
/
Productos del Gen env
/
Virus de la Inmunodeficiencia Felina
/
Formación de Anticuerpos
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
J Virol
Año:
1993
Tipo del documento:
Article
País de afiliación:
Italia