Inhibition of human cytomegalovirus major immediate early gene expression by antisense RNA expression vectors.
J Gen Virol
; 74 ( Pt 9): 1965-7, 1993 Sep.
Article
en En
| MEDLINE
| ID: mdl-8397287
ABSTRACT
We have used antisense oligonucleotides and expression vectors to inhibit human cytomegalovirus (HCMV) major immediate early (IE) gene expression. We find that oligonucleotides complementary to the HCMV 72K IE protein (IE1) coding region do inhibit HCMV infection, but this is non-specific. However, the use of certain antisense expression vectors, which express short oligonucleotides complementary to IE1, specifically inhibits IE1 expression at the protein level after introduction of IE expression vectors into cells or after HCMV infection.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Regulación Viral de la Expresión Génica
/
Oligonucleótidos Antisentido
/
ARN sin Sentido
/
Proteínas Inmediatas-Precoces
/
Citomegalovirus
Límite:
Humans
Idioma:
En
Revista:
J Gen Virol
Año:
1993
Tipo del documento:
Article
País de afiliación:
Reino Unido