Lectin binding and uptake and glycoprotein characterization of isolated porcine aortic endothelial and smooth muscle cells.
Cell Biochem Funct
; 11(3): 225-30, 1993 Sep.
Article
en En
| MEDLINE
| ID: mdl-8403237
ABSTRACT
Endothelial and smooth muscle cells were isolated from porcine aorta and kept in short-term culture. To determine the terminal carbohydrate composition of the plasma membranes from both cell populations, the cells were incubated with a panel of fluorescein-labelled lectins. Both cell populations shared a number of terminal carbohydrates, but the N-galactosamine specific lectin Wistaria floribunda agglutinin labelled only endothelial cells. A lectin which selectively labelled smooth muscle cells was not found. Western blot analysis of isolated endothelial cell membrane glycoproteins indicated that most membrane glycoproteins are labelled by Wistaria floribunda agglutinin.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Carbohidratos
/
Endotelio Vascular
/
Glicoproteínas de Membrana
/
Membrana Celular
/
Lectinas
/
Músculo Liso Vascular
Límite:
Animals
Idioma:
En
Revista:
Cell Biochem Funct
Año:
1993
Tipo del documento:
Article
País de afiliación:
Reino Unido