Divergent effects of interleukin-10 on cytokine production by mononuclear phagocytes and endothelial cells.
Eur J Immunol
; 23(10): 2692-5, 1993 Oct.
Article
en En
| MEDLINE
| ID: mdl-8405067
ABSTRACT
Interleukin-10 (IL-10), a product of T helper type 2 (TH2) cells and monocytes, inhibits cytokine production in mononuclear phagocytes. Given the similarities and interrelationship between cells of the monocyte-macrophage lineage and endothelial cells, we examined the effects of IL-10 on vascular endothelium. Murine IL-10 induced low levels of IL-6 production and amplified induction of IL-6 by lipopolysaccharide (LPS) or IL-1 in the murine tEND.1 endothelioma line, used for these studies because it retains properties of normal endothelium. The effect was more evident after prolonged (48-72 h) exposure to IL-10. IL-10 had similar activity on other endothelioma lines, whereas it inhibited IL-6 production by peritoneal macrophages. Induction and amplification of cytokine production by IL-10 was associated with higher levels of mRNA, which were maintained longer (up to 48 h) than in controls. In addition to IL-6, murine IL-10 induced or amplified expression of the chemoattractant cytokines monocyte chemotactic protein-1 (MCP-1) and KC. Human IL-10 inhibited IL-6 release by LPS-stimulated human peripheral blood mononuclear cells, whereas it did not interfere with cytokine production by LPS- or IL-1-stimulated human umbilical vein endothelial cells. The selective inhibitory action of IL-10 on mononuclear phagocytes versus endothelial cells may play a role in the pathophysiology of TH2-directed responses.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fagocitos
/
Endotelio Vascular
/
Interleucina-6
/
Interleucina-10
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Eur J Immunol
Año:
1993
Tipo del documento:
Article
País de afiliación:
Italia