Use of DNA end joining activity of a Xenopus laevis egg extract for construction of deletions and expression vectors for HIV-1 Tat and Rev proteins.
Gene
; 124(2): 275-80, 1993 Feb 28.
Article
en En
| MEDLINE
| ID: mdl-8444351
ABSTRACT
We have developed a cloning strategy which combines conventional T4 DNA ligation with the highly efficient nonhomologous DNA end joining (EJ) activity of an extract from Xenopus laevis eggs. The nonhomologous EJ activity allowed the rapid construction of deletion mutants by the intramolecular rejoining of nonhomologous DNA ends generated for the purpose of deleting restriction fragments from the vector. The combined use of T4 DNA ligase for intermolecular ligation and X. laevis egg extracts for intramolecular nonhomologous EJ proved to be a powerful tool, as demonstrated here for the construction of expression vectors for HIV-1 Tat and Rev.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Productos del Gen rev
/
Productos del Gen tat
/
VIH-1
/
Clonación Molecular
/
Eliminación de Secuencia
/
Vectores Genéticos
Límite:
Animals
Idioma:
En
Revista:
Gene
Año:
1993
Tipo del documento:
Article
País de afiliación:
Alemania