Size-exclusion chromatographic determination of beta-glucan with postcolumn reaction detection.
J Chromatogr
; 632(1-2): 105-10, 1993 Feb 19.
Article
en En
| MEDLINE
| ID: mdl-8454712
ABSTRACT
Using the specific interaction of beta-glucan with calcofluor dye as a detection method for size-exclusion chromatographic analysis, very simple direct extractions can be used for sample preparation. Either fluorescence or UV detection is employed. The calibration graphs are linear between 200 and 20 mg/l, although much more sensitive analyses are possible. The response remained independent of relative molecular mass in the range studied (1.7 x 10(6)-1.85 x 10(5)). Results for barley and malt show good correlation with those obtained by flow-injection analysis using calcofluor.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Cromatografía en Gel
/
Glucanos
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
J Chromatogr
Año:
1993
Tipo del documento:
Article