The straw swim-up: a simple method to evaluate the effects of additives, media, or toxicants on sperm motility.
Fertil Steril
; 59(6): 1322-4, 1993 Jun.
Article
en En
| MEDLINE
| ID: mdl-8495786
ABSTRACT
We describe a new method for evaluating potential toxicants, spermicides, and motility enhancers. Cryopreservation straws filled with media plus additive are emersed below the surface of an unprocessed donor ejaculate. After incubation, the sperm found within the first 4 cm of the straw are expelled and analyzed. The effects of media containing glycerol and albumin were examined. Survivability and sperm migration into the media occur independently and can be differentiated in this assay. This method allows for the use of unprocessed semen and includes an evaluation of the semen and media interaction.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Motilidad Espermática
Límite:
Humans
/
Male
Idioma:
En
Revista:
Fertil Steril
Año:
1993
Tipo del documento:
Article