Increased expression of apolipoprotein genes accompanies differentiation in the intestinal cell line Caco-2.
Proc Natl Acad Sci U S A
; 90(12): 5757-61, 1993 Jun 15.
Article
en En
| MEDLINE
| ID: mdl-8516325
ABSTRACT
We have analyzed determinants of the synthesis and secretion of apolipoproteins including mRNA for apolipoproteins, in the human colon carcinoma cell line Caco-2 during differentiation in continuous culture. Significant increases in both cellular and secreted apolipoprotein A-I were observed early in the differentiation process. Increases in apolipoprotein B were limited to secreted protein and started later in the differentiation process. Levels of mRNA for apolipoproteins A-I, A-IV, B, C-III, and E increased significantly between the time cells reached confluence and 1 week postconfluence. The kinetics of mRNA accumulation were influenced by culture conditions. Nuclear extracts from postconfluent Caco-2 cells contained increased amounts of protein that bound to oligonucleotides containing the control regions of the apolipoprotein A-I and B genes. A competition experiment suggested that this protein recognized the control regions of both genes. We propose to name this protein DRIFT-1 (differentiation-related intestinal factor for transcription 1).
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Apolipoproteínas
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ARN Mensajero
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Expresión Génica
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Diferenciación Celular
Límite:
Humans
Idioma:
En
Revista:
Proc Natl Acad Sci U S A
Año:
1993
Tipo del documento:
Article