Can the immunobead assay for detecting sperm antibodies in fresh samples be reproduced in cryopreserved/thawed human spermatozoa?
Am J Reprod Immunol
; 34(2): 116-9, 1995 Aug.
Article
en En
| MEDLINE
| ID: mdl-8526987
ABSTRACT
PROBLEM:
To evaluate the reproducibility of the Immunobead Assay (IBA) on sperm samples before and after cryopreservation.METHOD:
Sperm samples (fresh and post-thaw) from known antibody negative donors (N = 20) were evaluated for percent immunobead binding by IBA following incubation with known antibody-positive serum.RESULTS:
In both fresh and thawed negative samples, the mean sperm head binding was 0.5% +/- 0.5, the mean sperm tail binding was 2.0% +/- 2.0 and the mean sperm head-tail binding was 3.0% +/- 2.0 for IgG, IgA and IgM type antibodies, respectively. The same samples exposed to positive sera showed 40.0% +/- 10.0 mean head binding, 7.0% +/- 8.0 mean tail binding and 47.0% +/- 11.0 mean head-tail binding.CONCLUSIONS:
IBA is highly reproducible for detecting sperm antibodies in both fresh and cryopreserved/thawed samples of human spermatozoa.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Autoanticuerpos
/
Espermatozoides
/
Inmunoensayo
/
Criopreservación
Límite:
Humans
/
Male
Idioma:
En
Revista:
Am J Reprod Immunol
Año:
1995
Tipo del documento:
Article
País de afiliación:
Estados Unidos