Integration of an Epstein-Barr virus episome 3' into the gene encoding immunoglobulin heavy-chain alpha 1 in a lymphoblastoid cell line.
Gene
; 166(2): 221-6, 1995 Dec 12.
Article
en En
| MEDLINE
| ID: mdl-8543165
ABSTRACT
For the first time we have characterized an unoccupied site of Epstein-Barr (EBV) virus integration in a lymphoblastoid cell line, RGN1. The site of integration is about 1.5 kb downstream from the gene encoding the heavy chain constant alpha 1, specifying immunoglobulin A (IgA). Sequence and Southern analysis allowed us to hypothesize that integration occurred via a double exchange involving the viral latent origin of DNA replication (oriP) and the human DNA. The region involved in the integration is transcribed into poly(A)+ RNA in all the tested lymphoid lines, but not in the RGN1 line. We suggest a mechanism of integration primed by interactions between oriP and cell ori and its potential role in the establishment and/or evolution of EBV-carrying lines.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Genes de Inmunoglobulinas
/
Integración Viral
/
Herpesvirus Humano 4
Límite:
Humans
Idioma:
En
Revista:
Gene
Año:
1995
Tipo del documento:
Article
País de afiliación:
Italia