Effect of catecholestrogens on [3H]-GABA uptake by hypothalamic crude synaptosomes.
Arch Physiol Biochem
; 103(4): 416-21, 1995 Aug.
Article
en En
| MEDLINE
| ID: mdl-8548475
ABSTRACT
Catecholestrogens (CE), 2-hydroxyestradiol, 2-hydroxyestrone and primary estrogens, estradiol and estrone were tested in their ability to compete for the high affinity uptake of [3H]-GABA into crude synaptosomal fractions. Aliquots of the crude synaptosomal fraction obtained from normal rats were incubated for 10 min at 37 degrees C with [3H]-GABA in the presence, or absence, of estrogens and catecholestrogens. Neither estradiol nor estrone modified the specific [3H]-GABA uptake into crude synaptosomal fractions. On the contrary, CE significantly affected the specific [3H]-GABA uptake in a dose-dependent manner low concentrations of CE enhanced the uptake; this effect disappeared with high concentrations of the compounds. The stimulatory effect of CE on [3H]-GABA uptake was blocked when samples were coincubated with nipecotic acid, thus suggesting that this effect is specific rather than the result of non-specific interactions of CE with the hypothalamic membrane.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Sinaptosomas
/
Prolina
/
Estrógenos de Catecol
/
Estradiol
/
Ácido gamma-Aminobutírico
/
Hidroxiestronas
/
Hipotálamo
Límite:
Animals
Idioma:
En
Revista:
Arch Physiol Biochem
Asunto de la revista:
BIOQUIMICA
/
FISIOLOGIA
Año:
1995
Tipo del documento:
Article
País de afiliación:
Argentina