Establishment of immortal hepatocytes from a CYP3A7-transgenic/p53-knockout mouse.

Shimoji, M; Hattori, K; Itoh, S; Nakayama, K; Katsuki, M; Aizawa, S; Yokoi, T; Kamataki, T

Shimoji, M; Hattori, K; Itoh, S; Nakayama, K; Katsuki, M; Aizawa, S; Yokoi, T; Kamataki, T.

Afiliación

Shimoji M; Division of Drug Metabolism, Faculty of Pharmaceutical Sciences, Hokkaido University, Japan.

Biochem Biophys Res Commun ; 217(3): 1001-5, 1995 Dec 26.

Article en En | MEDLINE | ID: mdl-8554550

RESUMEN

Immortal hepatocytes were established from a CYP3A7-transgenic/p53-knockout mouse. The cells could be maintained with shorter doubling time after culture for 1 month. Detectable amounts of CYP3A7 mRNA and the activity of 7-propoxycoumarin O-depropylase, one of the representative CYP3A activities, were present in the immortal hepatocytes. Albumin mRNA, which is specifically expressed in the liver, remained in these cells.

Asunto(s)

7-Alcoxicumarina O-Dealquilasa/genética; Sistema Enzimático del Citocromo P-450/genética; Hígado/citología; Animales; Células Cultivadas; Regulación del Desarrollo de la Expresión Génica; Genes p53; Humanos; Hígado/enzimología; Ratones; Ratones Noqueados; Ratones Transgénicos; ARN Mensajero/genética

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: 7-Alcoxicumarina O-Dealquilasa / Sistema Enzimático del Citocromo P-450 / Hígado Límite: Animals / Humans Idioma: En Revista: Biochem Biophys Res Commun Año: 1995 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos

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