Deficient signaling in mice devoid of double-stranded RNA-dependent protein kinase.
EMBO J
; 14(24): 6095-106, 1995 Dec 15.
Article
en En
| MEDLINE
| ID: mdl-8557029
ABSTRACT
Double-stranded RNA-dependent protein kinase (PKR) has been implicated in interferon (IFN) induction, antiviral response and tumor suppression. We have generated mice devoid of functional PKR (Pkr%). Although the mice are physically normal and the induction of type I IFN genes by poly(I).poly(C) (pIC) and virus is unimpaired, the antiviral response induced by IFN-gamma and pIC was diminished. However, in embryo fibroblasts from Pkr knockout mice, the induction of type I IFN as well as the activation of NF-kappa B by pIC, were strongly impaired but restored by priming with IFN. Thus, PKR is not directly essential for responses to pIC, and a pIC-responsive system independent of PKR is induced by IFN. No evidence of the tumor suppressor activity of PKR was demonstrated.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas Serina-Treonina Quinasas
Límite:
Animals
Idioma:
En
Revista:
EMBO J
Año:
1995
Tipo del documento:
Article
País de afiliación:
Suiza