Torulopsis glabrata: azole susceptibilities by microdilution colorimetric and macrodilution broth assays.
J Clin Microbiol
; 33(10): 2612-5, 1995 Oct.
Article
en En
| MEDLINE
| ID: mdl-8567892
ABSTRACT
Fluconazole and itraconazole MICs were determined by both the standard macrodilution method of the National Committee for Clinical Laboratory Standards and a colorimetric broth microdilution method for 140 isolates of Torulopsis (Candida) glabrata obtained over a 15-year period. Using the method of the National Committee for Clinical Laboratory Standards the MICs at which 90% of isolates are inhibited (MIC50) for all isolates were 32 and 1.6 micrograms/ml for fluconazole and itraconazole, respectively. For fluconazole, the MIC90 rose from 16 to > 64 micrograms/ml when the MIC90s for isolates collected from July 1980 to June 1991 were compared with those for isolates collected from July 1991 to March 1995. For itraconazole, the MIC90s for isolates from the same time periods were 0.8 and 3.2 micrograms/ml, respectively. Although for isolates from some non-human immunodeficiency virus-infected patients the MICs rose, most of the high MICs were found for isolates from human immunodeficiency virus-infected patients who had been extensively treated with azole drugs for thrush. The colorimetric method yielded endpoints that were more definitive; concordances within 2 dilutions for the two methods were 87% for fluconazole and 86% for itraconazole.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Candida
/
Candidiasis
/
Fluconazol
/
Itraconazol
/
Antifúngicos
Tipo de estudio:
Guideline
Límite:
Humans
Idioma:
En
Revista:
J Clin Microbiol
Año:
1995
Tipo del documento:
Article
País de afiliación:
Estados Unidos