[Isolation and characteristics of a new trypsin and chymotrypsin inhibitor from potato tubers]. / Vydelenie i kharakteristika novogo ingibitora tripsina i khimotripsina iz klubnei kartofelia.
Biokhimiia
; 60(11): 1844-52, 1995 Nov.
Article
en Ru
| MEDLINE
| ID: mdl-8590757
ABSTRACT
A novel trypsin and chymotrypsin inhibitor has been isolated from potato (Solanum tuberosum L.) tubers. The isolation procedure included ammonium sulfate precipitation, gel-chromatography on Sephadex G-75 and ion-exchange chromatography on DEAE-cellulose. The inhibitor interacts with trypsin and chymotrypsin at a molar ratio of 11. The substrate-dependent dissociation of the enzyme-inhibitor complexes is observed. The inhibitor displays no activity towards subtilisin and pancreatic elastase. The ability of the inhibitor to form a ternary complex containing simultaneously both trypsin and chymotrypsin molecules testifies to the presence of two independent reactive sites for these enzymes.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Quimotripsina
/
Inhibidores de Tripsina
Idioma:
Ru
Revista:
Biokhimiia
Año:
1995
Tipo del documento:
Article