The interferon-inducible growth-inhibitory p202 protein: DNA binding properties and identification of a DNA binding domain.
Biochem Biophys Res Commun
; 221(2): 396-401, 1996 Apr 16.
Article
en En
| MEDLINE
| ID: mdl-8619867
ABSTRACT
p202 is an interferon-inducible protein whose expression in transfected cells inhibits proliferation. p202 binds to the retinoblastoma tumor suppressor protein in vitro and in vivo and the transcription factors AP-1 c-Fos and c-Jun, NF-kappaB p50 and p65, and inhibits the transcriptional activity of these factors in vivo. Here we report that p202 nonspecifically binds to double-stranded DNA and to single-stranded DNA in vitro. Analysis with recombinant p202 revealed that DNA binding activity is intrinsic to p202. A C-terminal deletion mutant of p202 exhibited DNA-binding properties, indicating that the C-terminus is dispensable for DNA binding. We also found that underphosphorylated p202 efficiently binds to DNA. Our data suggest that DNA binding activity of p202 may contribute to its functions.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fosfoproteínas
/
Proteínas Portadoras
/
Interferones
/
Péptidos y Proteínas de Señalización Intracelular
/
Proteínas de Unión al ADN
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
1996
Tipo del documento:
Article
País de afiliación:
Estados Unidos