V beta 8.2 transgene expression interferes with development of experimental autoimmune thyroiditis in CBA k/q but not k/k mice.
Cell Immunol
; 168(2): 297-301, 1996 Mar 15.
Article
en En
| MEDLINE
| ID: mdl-8640878
ABSTRACT
The thyroiditogenic T cell receptor (TCR) repertoire is not yet well defined in murine experimental autoimmune thyroiditis (EAT). Our recent work has shown that, while V beta 8+ T cells have no major role in EAT induction with mouse thyroglobulin (MTg), V beta 13 may be involved. To examine the effect of skewing the TCR repertoire on EAT development, CBA (H2k) mice were mated with B10.Q mice harboring an ovalbumin-specific V beta 8.2 TCR transgene (trg), and the trg+ mice were backcrossed to CBA. FACS analysis showed that peripheral blood T cells from trg+ mice had about 76 and 90% V beta 8.2+ cells in the CD4+ and CD8+ subsets, respectively, compared with about 15 and 11% in trg- sibs. The transgenic CBA k/k and k/q mice were immunized with MTg and sacrificed 28 days later. In all trg+ mice, anti-MTg titers and T cell proliferative responses to MTg were significantly lowered. However, thyroid infiltration was distinctly different in the two strains of transgenic mice; a significant decrease was seen primarily in k/q, but not k/k, trg+ mice. Thus, skewing the TCR repertoire to overexpress an irrelevant TCR revealed the possession of a less flexible thyroiditogenic TCR repertoire in k/q, but not k/k, mice.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Enfermedades Autoinmunes
/
Tiroiditis Autoinmune
/
Antígenos H-2
/
Subgrupos de Linfocitos T
/
Receptores de Antígenos de Linfocitos T alfa-beta
/
Reordenamiento Génico de la Cadena beta de los Receptores de Antígenos de los Linfocitos T
/
Transgenes
/
Ratones Endogámicos CBA
Límite:
Animals
Idioma:
En
Revista:
Cell Immunol
Año:
1996
Tipo del documento:
Article
País de afiliación:
Estados Unidos