A peptide recognized by human cytolytic T lymphocytes on HLA-A2 melanomas is encoded by an intron sequence of the N-acetylglucosaminyltransferase V gene.
J Exp Med
; 183(3): 1173-83, 1996 Mar 01.
Article
en En
| MEDLINE
| ID: mdl-8642259
ABSTRACT
A cytolytic T lymphocyte (CTL) clone that lyses many HLA-A2 melanomas was derived from a population of tumor-infiltrating lymphocytes of an HLA-A2 melanoma patient. The gene coding for the antigen recognized by this CTL was identified by transfection of a cDNA library. It is the gene which has been reported to code for N-acetylglucosaminyltransferase V (GnT-V). Remarkably, the antigenic peptide recognized by the CTL is encoded by a sequence located in an intron. In contrast to the fully spliced GnT-V mRNA, which was found in a wide range of normal and tumoral tissues, the mRNA containing the intron region coding for the antigen was not found at a significant level in normal tissues. This mRNA was observed to be present in about 50% of melanomas. Our results suggest that a promoter located near the end of the relevant intron is activated in melanoma cells, resulting in the production of an mRNA coding for the antigen.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Intrones
/
Linfocitos T Citotóxicos
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Antígeno HLA-A2
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Linfocitos Infiltrantes de Tumor
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N-Acetilglucosaminiltransferasas
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Melanoma
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Antígenos de Neoplasias
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Neoplasias
Límite:
Female
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Humans
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Male
Idioma:
En
Revista:
J Exp Med
Año:
1996
Tipo del documento:
Article
País de afiliación:
Bélgica