Perturbation of nuclear architecture by long-distance chromosome interactions.
Cell
; 85(5): 745-59, 1996 May 31.
Article
en En
| MEDLINE
| ID: mdl-8646782
ABSTRACT
SUMMARY:
Position-effect variegation (PEV) describes the stochastic transcriptional silencing of a gene positioned adjacent to heterochromatin. Using FISH, we have tested whether variegated expression of the eye-color gene brown in Drosophila is influenced by its nuclear localization. In embryonic nuclei, a heterochromatic insertion at the brown locus is always spatially isolated from other heterochromatin. However, during larval development this insertion physically associates with other heterochromatic regions on the same chromosome in a stochastic manner. These observations indicate that the brown gene is silenced by specific contact with centromeric heterochromatin. Moreover, they provide direct evidence for long-range chromosome interactions and their impact on three-dimensional nuclear architecture, while providing a cohesive explanation for the phenomenon of PEV.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Núcleo Celular
/
Cromosomas
/
Genes de Insecto
/
Drosophila
Límite:
Animals
Idioma:
En
Revista:
Cell
Año:
1996
Tipo del documento:
Article
País de afiliación:
Estados Unidos