[Dot-blot analysis in laboratory diagnosis of hemorrhagic fever with renal syndrome]. / Dot-blot analiz v laboratornoi diagnostike gemorragicheskoi likhoradki s pochechnym sindromom.
Vopr Virusol
; 41(1): 6-8, 1996.
Article
en Ru
| MEDLINE
| ID: mdl-8669150
ABSTRACT
A sensitive micro EIA utilizing antigen or antibody dotted onto nitrocellulose filters (Dot-ELISA, Dot-blot) has been developed for laboratory diagnosis of hemorrhagic fever with the renal syndrome (HFRS) by detecting the virus specific IgM and IgG in the sera of HFRS patients and the antigen in crude lung suspensions of wild rodents. Measurements of specific IgM and IgG in paired HFRS sera collected during the first month of the disease showed clear-cut seroconversion and 100% correlation with the results of immunofluorescent test. The results of antigen detection in lung suspensions of 605 wild rodents trapped in various regions of Russia were identical to standard ELISA results. This rapid and inexpensive test may be useful in early serologic diagnosis of HFRS and in field serological and epizootological studies.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ensayo de Inmunoadsorción Enzimática
/
Fiebre Hemorrágica con Síndrome Renal
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
/
Humans
Idioma:
Ru
Revista:
Vopr Virusol
Año:
1996
Tipo del documento:
Article