[An effective method of purifying recombinant human tumor necrosis factor alpha]. / Effektivnyi metod ochistki rekombinantnogo alpha-faktora nekroza opukholei cheloveka.
Bioorg Khim
; 22(3): 163-7, 1996 Mar.
Article
en Ru
| MEDLINE
| ID: mdl-8687507
ABSTRACT
An efficient and productive isolation method for human recombinant tumor necrosis factor alpha from Escherichia coli cells was developed. The method includes a membrane filtration step, two steps of ion-exchange chromatography, and gel filtration on a Sephadex G-25 column. The target product was obtained with approximately 50% total yield and greater than 95% purity according to PAGE and HPLC.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Factor de Necrosis Tumoral alfa
Límite:
Humans
Idioma:
Ru
Revista:
Bioorg Khim
Año:
1996
Tipo del documento:
Article