Active heterodimers are formed from human DNA topoisomerase II alpha and II beta isoforms.
Proc Natl Acad Sci U S A
; 93(16): 8288-93, 1996 Aug 06.
Article
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| MEDLINE
| ID: mdl-8710863
ABSTRACT
DNA topoisomerase II is a nuclear enzyme essential for chromosome dynamics and DNA metabolism. In mammalian cells, two genetically and biochemically distinct topoisomerase II forms exist, which are designated topoisomerase II alpha and topoisomerase II beta. In our studies of human topoisomerase II, we have found that a substantial fraction of the enzyme exists as alpha/beta heterodimers in HeLa cells. The ability to form heterodimers was verified when human topoisomerases II alpha and II beta were coexpressed in yeast and investigated in a dimerization assay. Analysis of purified heterodimers shows that these enzymes maintain topoisomerase II specific catalytic activities. The natural existence of an active heterodimeric subclass of topoisomerase II merits attention whenever topoisomerases II alpha and II beta function, localization, and cell cycle regulation are investigated.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
ADN-Topoisomerasas de Tipo I
Límite:
Humans
Idioma:
En
Revista:
Proc Natl Acad Sci U S A
Año:
1996
Tipo del documento:
Article
País de afiliación:
Dinamarca