Trichomonas vaginalis haemolysis: pH regulates a contact-independent mechanism based on pore-forming proteins.
Microb Pathog
; 20(2): 109-18, 1996 Feb.
Article
en En
| MEDLINE
| ID: mdl-8722099
ABSTRACT
There is a controversy in literature about involvement of secreted factors in the pathogenetic mechanisms of Trichomonas vaginalis, described mostly as contact-dependent. We found that the protozoan, under triggering conditions, is able to release molecules that lead to lysis without direct contact between parasite and target cells as a prerequisite. In this paper we characterize contact-independent cytotoxicity using the red blood cell as a cellular model. Contact-independent haemolysis is a phenomenon were pH exerts a key role, triggering the secretion of a lytic molecule and regulating its activity. A partial physicochemical characterization of the haemolytic factor suggests that a protein of M(r) > 30 kDa could be the effector responsible for damage. Furthermore, the parasite-induced membrane permeabilization, detected by measuring potassium escape from the target cell, and an effective osmotic protection by carbohydrates allowed us to relate the previously described pore-forming mechanism involved in contact-dependent cytotoxicity with the contact-independent lysis.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Trichomonas vaginalis
/
Porinas
/
Hemólisis
/
Concentración de Iones de Hidrógeno
Tipo de estudio:
Prognostic_studies
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Microb Pathog
Asunto de la revista:
DOENCAS TRANSMISSIVEIS
/
MICROBIOLOGIA
Año:
1996
Tipo del documento:
Article
País de afiliación:
Italia