Interleukin-1 beta stimulates glucose uptake of human peritoneal mesothelial cells in vitro.
Perit Dial Int
; 16 Suppl 1: S58-60, 1996.
Article
en En
| MEDLINE
| ID: mdl-8728164
ABSTRACT
To investigate whether the glucose uptake (GU) of human peritoneal mesothelial cells (HPMC) is mediated by glucose transporters and whether this uptake is influenced by interleukin 1-beta (IL-1 beta), we measured 2-deoxy-(3H)-GU of HPMC in vitro, after exposing the cells for different times (two and 12 hours) to increasing concentrations (0.1, 1.0, and 2.0 ng/mL) of IL-1 beta. To exclude a noncarrier-mediated transport, GU was also tested in the presence of cytochalasin B. All experiments were performed in triplicate in the cells of two donors. Cytochalasin B inhibits GU of HPMC almost completely. GU of HPMC is not stimulated by insulin. GU is stimulated by IL-1 beta in a dose-dependent manner. These data indicate a GU of HPMC, which is mediated by a glucose transporter and stimulated by IL-1 beta. The increased uptake of glucose from the dialysate in patients with peritonitis may be mediated by a (cytokine-induced) increased activity of HPMC glucose transporters.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Peritoneo
/
Glucemia
/
Interleucina-1
Límite:
Humans
Idioma:
En
Revista:
Perit Dial Int
Asunto de la revista:
NEFROLOGIA
Año:
1996
Tipo del documento:
Article
País de afiliación:
Alemania