The sequence of a 24,152 bp segment from the left arm of chromosome XIV from Saccharomyces cerevisiae between the BNI1 and the POL2 genes.
Yeast
; 12(5): 505-14, 1996 Apr.
Article
en En
| MEDLINE
| ID: mdl-8740425
ABSTRACT
In the framework of the European Union programme for sequencing the genome of Saccharomyces cerevisiae we have determined the nucleotide sequence of a region of 24,152 bp located on the left arm of chromosome XIV between the BNI1 and the POL2 genes. The sequence was obtained by directed sequence analysis using a mixture of ExoIII and primer walking strategies. Subsequent analysis revealed 13 open reading frames (ORFs) including four small ORFs completely internal to, or partly overlapping with, other ORFs. Five of these ORFs have been described previously (BNI1, APL1, LYP1, PIK1, POL2) and thus 74.8% of the 24,152 bp were already present in the databases prior to this sequencing effort. Interestingly, all 13 identified ORFs are characterized by a low codon adaptation index (0.04-0.22). In addition, this region of chromosome XIV shows an unusually high gene density with about 88% of coding DNA. This amounts to one gene per 2177 bp, which is significantly above the average gene length (about 1500 bp). For eight ORFs considerable homologies to 'Expressed Sequence Tags' derived from human cDNAs located in the XREF database could be identified.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Saccharomyces cerevisiae
/
Cromosomas Fúngicos
/
Sistemas de Lectura Abierta
Límite:
Humans
Idioma:
En
Revista:
Yeast
Asunto de la revista:
MICROBIOLOGIA
Año:
1996
Tipo del documento:
Article
País de afiliación:
Alemania