The effects of hemodilution with polyethylene glycol bovine hemoglobin (PEG-Hb) in a conscious porcine model.
J Investig Med
; 44(5): 238-46, 1996 Jun.
Article
en En
| MEDLINE
| ID: mdl-8763974
ABSTRACT
BACKGROUND:
Severe hemodilution in large mammals has been used rigorously for the safety and efficacy testing of hemoglobin-based red blood cell substitutes. The effects of hemodilution with polyethylene glycol-modified bovine hemoglobin (PEG-Hb) were investigated in an unanesthetized porcine model.METHODS:
Immature Yorkshire cross barrow pigs were subjected to exchange transfusion with PEG-Hb (n = 6) or dextran 70 (n = 4) until an 80% reduction in hematocrit was achieved.RESULTS:
All six (100%) PEG-Hb-infused pigs and only one (25%) dextran 70 control pig survived the resultant reduction in erythrocytes. Heart rates and mean arterial pressure were not significantly affected by PEG-Hb infusion. Pigs infused with PEG-Hb maintained normal levels of blood pH, PO2, and PCO2 while dextran 70 controls showed low PvO2, PaCO2, and the development of acidosis. Histological evaluation revealed that the surviving dextran 70 control animal exhibited possible anoxia-induced hepatic centrilobular necrosis. PEG-Hb-treated pigs demonstrated the presence of renal tubular cell cytoplasmic vacuoles and vacuolated macrophages in spleens.CONCLUSIONS:
The results indicate that PEG-Hb effectively supports life close to lethal levels of anemia.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Polietilenglicoles
/
Hemoglobinas
/
Hemodilución
Límite:
Animals
Idioma:
En
Revista:
J Investig Med
Asunto de la revista:
MEDICINA
Año:
1996
Tipo del documento:
Article
País de afiliación:
Estados Unidos