Isolation of tetranucleotide repeat polymorphisms flanking the BRCA1 gene.
Genomics
; 32(1): 163-7, 1996 Feb 15.
Article
en En
| MEDLINE
| ID: mdl-8786111
ABSTRACT
Large pools of cosmids from the BRCA1 region of human chromosome 17 were screened for tetranucleotide repeat polymorphisms by hybridizing shotgun subcloned pools with a mixture of 25 oligonucleotides. Identified subclones were PCR amplified and directly sequenced to design PCR primers for short tandem repeat polymorphism (STRP) analysis of family DNAs. With the identification of the BRCA1 gene and the observation that most mutations in this > 100-kb gene are unique, haplotyping and linkage analysis may play a significant role in diagnosis and carrier detection of BRCA1-associated breast and ovarian cancers. We report the characterization of 15 new STRPs flanking the BRCA1 locus.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Polimorfismo Genético
/
Factores de Transcripción
/
Genes Supresores de Tumor
/
Repeticiones de Microsatélite
/
Proteínas de Neoplasias
Tipo de estudio:
Prognostic_studies
Límite:
Female
/
Humans
Idioma:
En
Revista:
Genomics
Asunto de la revista:
GENETICA
Año:
1996
Tipo del documento:
Article
País de afiliación:
Estados Unidos