Detection of immobilized amplicons by ELISA-like techniques.
Clin Chem
; 42(9): 1547-55, 1996 Sep.
Article
en En
| MEDLINE
| ID: mdl-8787728
ABSTRACT
The NucleoLink surface is a physically modified, thermostable, optically clear resin. It allows the covalent binding of 5'-phosphorylated oligonucleotides. Target DNA amplification by polymerase chain reaction (PCR) is accomplished by asymmetric amplification on the covalently immobilized primer that develops into immobilized amplicons. A DNA fragment of bovine leukemia virus is used as a model system for the detection of immobilized amplicons by ELISA-like techniques. Covalently bound oligonucleotides are also utilized as capture probe in the hybridization-based signal amplification for detection of an infectious organism.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ensayo de Inmunoadsorción Enzimática
/
Reacción en Cadena de la Polimerasa
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Clin Chem
Asunto de la revista:
QUIMICA CLINICA
Año:
1996
Tipo del documento:
Article
País de afiliación:
Estados Unidos