Thi1, a thiamine biosynthetic gene in Arabidopsis thaliana, complements bacterial defects in DNA repair.
Plant Mol Biol
; 31(3): 585-93, 1996 Jun.
Article
en En
| MEDLINE
| ID: mdl-8790291
ABSTRACT
An Arabidopsis thaliana cDNA was isolated by complementation of the Escherichia coli mutant strain BW535 (xth, nfo, nth), which is defective in DNA base excision repair pathways. This cDNA partially complements the methyl methane sulfonate (MMS) sensitive phenotype of BW535. It also partially corrects the UV-sensitive phenotype of E. coli AB1886 (uvrA) and restores its ability to reactivate UV-irradiated lambda phage. It has an insert of ca. 1.3 kb with an open reading frame of 1047 bp (predicting a protein with a molecular mass of 36 kDa). This cDNA presents a high homology to a stress related gene from two species of Fusarium (sti35) and to genes whose products participate in the thiamine biosynthesis pathway, THI4, from Saccharomyces cerevisiae and nmt2 from Schizosaccharomyces pombe. The Arabidopsis predicted polypeptide has homology to several protein motifs amino-terminal chloroplast transit peptide, dinucleotide binding site, DNA binding and bacterial DNA polymerases. The auxotrophy for thiamine in the yeast thi4URA3 disruption strain is complemented by the Arabidopsis gene. Thus, the cloned gene, named thi1, is likely to function in the biosynthesis of thiamine in plants. The data presented in this work indicate that thi1 may also be involved in DNA damage tolerance in plant cells.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Tiamina
/
Factores de Transcripción
/
Rayos Ultravioleta
/
Genes de Plantas
/
Arabidopsis
/
Bacteriófago lambda
/
Proteínas de Arabidopsis
/
Proteínas de Unión al ADN
/
Reparación del ADN
/
Escherichia coli
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Plant Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
/
BOTANICA
Año:
1996
Tipo del documento:
Article
País de afiliación:
Brasil